info@shantani.com | Tel: +91-20-64103918, +91-20-64009991


 

What Do We Offer?

Shantani offers a comprehensive set of Cell-Biology and Cell-Based Assay Services. We have a fully functional cell-biology lab and over the last several years we have developed many cell-based assays for measuring biological activities of small-molecules and biologics. Following is a list of cell-based assay that we can customize or provide on routine basis.

  1. Cytotoxicity Assays
  2. Cytotoxicity or Cell Viability assays are widely used for screening drug candidates and testing cytotoxicity levels of bio-chemicals. These assays provide sensitive and efficient approach to quantify harmful dose ranges of compounds and analyze their effect on cell health. Any of the parameter(s) such as cell membrane integrity, redox potential of cell population, ATP/ADP levels and activity of cellular enzymes can be used as the basis of cytotoxicity or cell viability assay.

    We use a redox indicator dye Alamar blue for studying cytotoxicity levels in different type of cell lines. Alamar blue is a non-toxic indicator that can both fluoresces and changes color in response to the chemical reduction of growth medium resulting from cell growth. End point measurements are done using both colorimetry and fluorimetry. The assay is scalable and can be performed in different plate (6/12/24/96/384 wells) formats. Spectroscopic measurements are done using Synergy H1 Hybrid multi-well plate reader.

  3. Intracellular Calcium Measurement Assay
  4. Calcium plays an important role in cell signaling and acts as an important secondary messenger. Measurement of calcium influx is often used for screening GPCRs that causes changes in membrane potential. Several fluorescent calcium indicator dyes such as Fluo-3, Fluo-4 AM, Fluo-4 NW, Indo-1 and Fura-2 are available that can be used to measure intracellular calcium depending on specific experiment requirement and type of cell lines used.

    We use fluo-4 calcium indicator dye to measure calcium influx in the cells. Fluo-4 AM is a cell permeant visible-light fluorescent dye that can enter the cells with the help of acetomethyl ester group, which after entering in the cells gets cleaved by cellular esterase’s and binds with the available calcium to give fluorescence. The assay is scalable and can be performed in different plate (6/12/24/96/384 wells) formats. Fluorescence is acquired using Synergy H1 Hybrid multi-well plate reader.

  5. Insulin Secretion Measurement Assay
  6. Insulin regulates a wide spectrum of biological actions and plays a key role in regulating glucose homeostasis. Insulin secretion measurements are often performed to study the physiology of beta cells and the effect of potential anti-diabetic drug molecules on them.

    We use ELISA based method to study insulin secretion from relevant cell-lines. The cells are seeded in desired numbers and allowed to proliferate. The cells are than equilibrated with low glucose followed by stimulation with test molecules and/or high glucose. The amount of insulin released over a defined period of stimulation time is measured using standard Insulin ELISA kits. This assay can be performed in 24 well and 96 well plate formats.

  7. Nitrite oxide measurement Assay
  8. Nitric oxide is an important physiological messenger and effector molecule in many biological systems. It plays an important role in vascular regulation, neurotransmission, immune response and apoptosis. The measurement of nitrate/nitrite concentration is routinely used as an index of NO production.

    We use Griess reagent for quantitative analysis of nitrite (NO2–) in culture solutions. It is a colorimetric reagent that utilizes a diazotization chemical reaction (sulfanilamide and N-1-naphthylethylenediamine dihydrochloride under acidic conditions) to detect nitrite formed by the spontaneous oxidation of NO under physiological conditions. The assay is scalable and can be performed in different plate (6/12/24/96/384 wells) formats. Spectrophotometric measurements are performed on Synergy H1 Hybrid multi-well plate reader.

  9. Immunocytochemistry
  10. A visualization technique that is used to assess the presence of specific protein or antigen in cells by using a biomolecule such as a fluorophore, fluorescent dye, or an enzyme that is capable of binding to the protein of interest. The fluorescence or colorimetric signals from the reporter is detected using a microscope. The technique is widely used to study protein-protein interaction, proteins expression levels and other phenotypes.

High quality light-microscopy, fluorescence-microscopy, confocal-microscopy and Flow-cytometry analysis services can also be provided on need basis. Our dedicated team of experts will work with you and help you custom design the assays that will be best suited to your research needs.